Novogene 10x Single Cell RNA Sequencing: Project Experience Showcase Series 3

Novogene 10x Single Cell RNA Sequencing: Project Experience Showcase Series 3

Novogene San Jose lab is dedicated to offering comprehensive single cell RNA sequencing services for respected researchers. We aim to assist in decoding the transcriptome profile and immune repertoires, cell-by-cell, in an affordable, cost-effective, and customer-focused manner.

Powered by Next GEM technology from 10x Genomics and staffed by professional technicians and researchers, the Novogene San Jose lab has successfully performed a considerable number of 10x single cell/nuclei RNA sequencing projects on both regular and sophisticated tissue types, thus accumulating valuable project experience. We are now excited to share our project experiences in single nuclei RNA sequencing from frozen mouse liver tissues with you.

Frozen Mouse Liver Tissue Project Experience Up to June 2024

Based on commercial project data up to June 2024, the San Jose lab has received around a hundred of frozen mouse liver tissue samples shipped from all over the US. Approximately 96% of these samples have passed Novogene’s sample Quality Control (QC) and successfully progressed to library preparation and subsequent sequencing.

Project Workflow

Exemplified Single Nuclei Preparation Results

Pre-Debris Removal Microscope Image

Post-Debris Removal Microscope Image

Post-Debris Removal Luna Image

After the debris removal process, the isolated single nuclei meet the Quality Control (QC) criteria. The majority of nuclei exhibit standard morphology, with fewer than 20% showing deformities, slight debris, or clumping. The sample contains a sufficient concentration of nuclei, with an average viability of 0.35%.

Basic Bioinformatic Analysis Results by Cell Ranger

Based on Cell Ranger analysis, the estimated number of cells is 4,797, aligning closely with the client’s original target of 5,000 cells. A minimum of 50 mg of mouse liver frozen tissue is recommended to ensure an adequate number of target cells.

Novogene Powered Literatures Related to Liver Cells

Novogene Corporation Inc.

2921 Stockton Blvd. Suite 1810, Sacramento CA 95817

916-252-0068 ext. 383 inquiry_us@novogene.com www.novogene.com