Isoform Sequencing with Kinnex full-length RNA kit

Isoform Sequencing with Kinnex Full-Length RNA Kit

Based on the multiplexed arrays sequencing (MAS-Seq) method, the Kinnex™ full-length RNA kit concatenates smaller amplicons into larger fragment libraries for increased throughput. When paired with isoform sequencing (Iso-Seq) on the PacBio Revio platform, we achieve high-quality full-length transcript sequences, offering a remarkable 16-fold improvement over the regular method.

The Novogene Advantage

Enhanced Data Delivery: We deliver HiFi reads for Iso-Seq with the Kinnex kit.
Accelerated Turnaround Time: Equipped with five PacBio Revio platforms, we offer faster turnaround time.
Comprehensive Analysis: Our upgraded analysis process incorporates SQANTI3 for structural analysis, coupled with IsoQuant3.3 for in-depth gene expression analysis.

Project Workflow

Specifications

1. Sample Requirements:

*NC/QC = NanoDrop concentration/Qubit concentration

2. Library Type: Kinnex full-length cDNA library

3. Sequencing Platform: PacBio Revio platform

4. Recommended Data Output: 5M reads per sample for isoform discovery. 10M reads per sample for differential expression analysis. More reads may be required for particularly rare isoforms.

Analysis Pipeline

Novogene Corporation Inc.

2921 Stockton Blvd. Suite 1810, Sacramento CA 95817

916-252-0068 ext. 383 inquiry@novogeneusa.com www.novogene.com