RNA Sequencing | Sample-to-Insight with NovoMagic Plus

RNA Sequencing:

From Sample to Insight

An end-to-end RNA-Seq workflow, with RNA extraction fully integrated.

We are pleased to offer complimentary NovoMagic Plus access with every new bioinformatics analysis project.

Request a Quote Explore Bioinformatics Support

Every sample in your lab holds a story waiting to be told. We provide an integrated RNA workflow to help you uncover it. From high-quality RNA extraction across human and animal tissues, cell pellets, blood, and plant samples, to bioinformatics analysis on NovoMagic Plus, our pipeline connects each step, clearly and reliably.

You stay focused on discovery. We support the technical execution, experimental design, and downstream analysis to deliver results you can move forward with.

RNA Extraction Pricing & Sample Types

Our RNA extraction is performed using RNA-Seq optimised protocols to ensure consistent quality and reliable downstream analysis.

Expert Experimental Design Guidance

Beyond sample preparation and pricing, experimental design plays a critical role in determining the quality and interpretability of RNA-Seq results. For robust differential expression analysis, biological replication has a greater impact on statistical power than sequencing depth alone.

We recommend a minimum of six biological replicates, paired with an appropriate sequencing depth based on genome size (typically 30–50 million reads per sample for larger genomes). This balanced approach improves the detection of true differentially expressed genes while maintaining statistical reliability.

Not sure how to apply these recommendations to your study? Use the guide below as a starting point.

*If you are unable to obtain 6 biological replicates, we recommend a minimum of 3 biological replicates.

Speak with our team for project specific advice

These recommendations are guidelines and may be adjusted based on study objectives and sample availability.

References:

Illumina. (2023). Considerations for RNA Seq Read Length and Coverage - Illumina Knowledge. Illumina.com. https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000001243
Khetani, M. P., Meeta Mistry, Radhika. (2019, June 20). Experimental design considerations. Introduction to RNA-Seq Using High-Performance Computing - ARCHIVED. https://hbctraining.github.io/Intro-to-rnaseq-hpc-salmon/lessons/experimental_planning_considerations.html
Lamarre, S., Frasse, P., Zouine, M., Labourdette, D., Sainderichin, E., Hu, G., Le Berre-Anton, V., Bouzayen, M., & Maza, E. (2018). Optimization of an RNA-Seq Differential Gene Expression Analysis Depending on Biological Replicate Number and Library Size. Frontiers in plant science, 9, 108. https://doi.org/10.3389/fpls.2018.00108
Liu, Y., Zhou, J., & White, K. P. (2014). RNA-seq differential expression studies: more sequence or more replication?. Bioinformatics (Oxford, England), 30(3), 301–304. https://doi.org/10.1093/bioinformatics/btt688
Robles, J. A., Qureshi, S. E., Stephen, S. J., Wilson, S. R., Burden, C. J., & Taylor, J. M. (2012). Efficient experimental design and analysis strategies for the detection of differential expression using RNA-Sequencing. BMC genomics, 13, 484. https://doi.org/10.1186/1471-2164-13-484
Schurch, N. J., Schofield, P., Gierliński, M., Cole, C., Sherstnev, A., Singh, V., Wrobel, N., Gharbi, K., Simpson, G. G., Owen-Hughes, T., Blaxter, M., & Barton, G. J. (2016). How many biological replicates are needed in an RNA-seq experiment and which differential expression tool should you use?. RNA (New York, N.Y.), 22(6), 839–851. https://doi.org/10.1261/rna.053959.115

Bioinformatics Support with NovoMagic Plus

NovoMagic Plus enables you to turn RNA-Seq data into biological insight, enabling you to visualise, customise, and reanalyse results within a single platform. Users can customise reports, identify differentially expressed genes, and explore functional insights to support biological interpretation.

Some examples of visualizations:

Distribution of Gene Expression

GO Enrichment Analysis

KEGG Enrichment Analysis

Differential Gene Expression (DGE) Volcano Plot

Built for eukaryotic mRNA-Seq projects (human, plant, and animal), the platform integrates established resources such as Gene Ontology, KEGG, and STRING to support reliable, publication-ready analyses.

Our team can assist you with result interpretation and custom analysis workflows using NovoMagic Plus.

Discuss NovoMagic Plus for your project

A Transparent, Traceable Sample Journey

Track your project from sample receipt through library preparation, sequencing, and data delivery with clear visibility at every stage.

How we support your project:

Every step of your workflow is fully traceable, supported by CCTV monitoring for transparency and quality assurance.
Real-time sample status and project updates are available anytime through our Customer Service System (CSS), keeping project management simple and accessible.
Integrated access to NovoMagic Plus, enables downstream bioinformatics analysis once your data is delivered.

Discuss Your RNA-Seq Project & Pricing Options

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Terms and Conditions

The free NovoMagic Plus analysis promotion is exclusively available for all new mRNA projects that include reference-based analysis.
Eligibility requires a confirmed purchase order for Novogene library preparation and sequencing services. Prospective orders qualify.
Project quotes, purchase orders and physical samples must be received by June 30, 2026 to qualify for the promotion.
This exclusive offer cannot be combined with any other discounts or promotions from current ongoing Novogene campaigns.
Terms and conditions are subject to change without prior notice. Other restrictions may apply.

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About Novogene

Novogene is a pioneer in applying cutting-edge molecular biology technology and high-performance computing to research in the fields of life science and human health. Our vision is to continue as a global leader in the delivery of genomics services and solutions. With one of the largest sequencing capacities in the world, we utilise our deep scientific knowledge, first-class customer service and unsurpassed data quality to help clients realise their research goals in the rapidly evolving world of genomics. Novogene is committed to become your trusted genomics partner.

For more information, visit novogene.com/amea-en